Campaign Update Winter 2018

Welcome to another Campaign Update, keeping you informed of our constant fight to make things better for bunnies.

Richard’s new qualification

As if Richard Saunders was not already fantastic enough, he has added another qualification to his name. He now has the European College of Zoological Medicine, Diploma Specialist in Zoo Health Management to add to his accolades. This gives him more letters after his name, and he is now on the Scientific Committee for EBVS.

RVHD2 HIPRA webinar

Richard has recorded a webinar with HIPRA, who are the manufacturer of Eravac, on the ever-popular subject of RVHD2. As soon as it is available we will share the link to it on social media, so keep your eyes peeled.

Vet list

We now have over 110 rabbit friendly vets on the rabbit friendly vet list! This is free to access to anyone via our website. Due to huge demand we have had to close applications for the rest of 2018, because we have such a backlog to get through, but we will be opening it up again in the New Year. This is great news as it shows that practices are keen to be considered rabbit savvy, and realise the rising status of rabbits in the UK. Anyone who is looking for a rabbit savvy vet can find our list here: https://rabbitwelfare.co.uk/rabbit-care-advice/rabbit-friendly-vets/rabbit-friendly-vet-list/

Save the dates

We are finalising the conference dates for 2019 so you might like to save these dates:
1st June– Non clinical day (owners, rescue workers) in Birmingham.
1st June– Clinical͚Rabbit Essentials͛ day, in Birmingham. This is for vets and vet nurses. It is lecture based and will cover subjects that we think are essential for every small animal practice.
22nd June– Advanced Rabbit Practice, at The Royal Veterinary College, Hawkshead. Lecture based, but covering more advanced rabbit care and investigation using the fantastic team at the RVC.
23rd June– Rabbit Interactive Roadshow – 2 x 3-hour workshops covering dentistry and airway management, also at the RVC, with the fabulous Craig Hunt. Small group so be sure to book early.
1st December– Rabbit Interactive Roadshow – 2 x 3-hour workshops covering dentistry and airway management, in Newcastle Upon Tyne, using the awesome Kevin Eatwell. Small group so book early to grab a place.
Full details will follow shortly. Please keep an eye on our website, and social media, or sign up to our First Alert service.  You will be able to book via our shop website shortly.

Consultations and new legislation

Despite the amount of work and debate that Brexit has generated, there is still some progress with animal welfare legislation. There have been consultations for the UK for animal sentience and for Scotland with regards to breeding and licencing. This is obviously an area we are very keen on, given our Capone Campaign work, and something we can respond to with a lot of confidence. There have also been consultations on licencing of pet shops, riding schools etc., and on the 1st October 2018 the English government launched new regulations for the sale of puppies in the UK. This is great news, and we will be looking into the possibility of this legislation applying to rabbits also.

BBC Radio Shropshire – The rabbit and guinea pig debate

BBC Radio Shropshire phoned the helpline in October after an on air discussion between listeners who were discussing keeping rabbits and guinea pigs together, which caused some contention. We were invited on the next day to put the record straight, which is exactly what Richard did!

For interest, this is our official stance:

We are often asked about keeping rabbits and guinea pigs together. This is not advisable for the following reasons: ͞Bordetella bronchiseptica is a bacterial infection of the upper respiratory tract of a number of species, including dogs, rabbits and guinea pigs, related to B pertussis, which causes whooping cough in humans. It is often described as commensal in rabbits (i.e. found in this species without causing harm), however, it can be a primary disease-causing organism, and can complicate other infections such as Pasteurella. It can, though, be fatal in guinea pigs, and so keeping them in the same airspace as rabbits is not advised.
͞Rabbits and guinea pigs have different dietary requirements, particularly guinea pigs’ need for Vitamin C. ͞Rabbits and guinea pigs are not the same species, and cannot respond appropriately to one another’s behaviours. This may result in inadequate social behaviours, up to and including severe bullying. ͞
The main reason these species used to be kept together was for companionship without the risk of pregnancy. With improvements in anaesthetic safety and more widespread neutering of both species, this is less of a problem now. Whilst we would not recommend putting them together in the first place in this day and age, we would not advocate splitting up a stable sole rabbit:sole guinea pig pairing͟.

Please share our new vaccination poster

New vaccination poster

 

We are still hearing of owners who do not know about RVHD2 and the need for a second vaccine. Feedback suggested that our vaccination poster did not get the message across so we have a new vaccination poster which we hope will be more effective. If you are on social media please share. You can find it on our own social media pages and website. Just to clarify, this is our advice on vaccinations: ͞You will need to give your rabbits two vaccines every year to protect them. The most common are Nobivac (protects against myxomatosis and RVHD1) and Filavac (protects against RVHD1&2), or Eravac (protects against RVHD2)͟.

A full size version of the poster is on our Campaign page, under Resources

Latest on RVHD2

In addition to the confusion over the vaccines there seem to be rumours surfacing about more diseases. Just to put the record straight we have released this statement:

͞”Here at the RWAF we are aware of 3 significant fatal viral diseases of rabbits in the UK. ͞

Myxomatosis (covered by the vaccine Nobivac Myxo-RHD); Rabbit Viral Haemorrhagic Disease 1 (covered by Nobivac Myxo-RHD), and Rabbit Viral Haemorrhagic Disease 2 (covered by the vaccines Filavac KC and V, or Eravac).

͞We are not aware of any further versions of RVHD present in the UK, although the variant K5 has been discovered in Asia and Australasia. ͞

We are not aware of any viral infections that are acutely fatal to rabbits and rodents recently arriving in the UK. ͞

If anyone has documentary evidence of any exotic diseases arriving in the UK in future please inform us and the Animal and Plant Health Agency (APHA)͟.

A number of causes are being explored to explain the sudden deaths of numerous hares-(R Hale)

Myxomatosis in hares

Just as we were going to press there was an article in the news about a hare being diagnosed with myxomatosis in the UK.

Here at the RWAF we have been asked, over the past week or so, about myxomatosis in native wild brown hares in the UK. It’s important to be aware that this information is subject to change as the investigating continues, but is correct at time of posting.

Sporadic cases have been reported in the past, of suspected or confirmed myxomatosis in hares, including one which was written up in the veterinary press in 2014. However, this appears to be different in that multiple cases, from a wide geographical spread, are being reported to Dr Diana Bell, University of East Anglia and, whilst some have obvious external symptoms of myxomatosis, other dead hares look fine/in good condition or are seen dying with unusual neurological symptoms including an inability to move and seizures. A number of possible causes are being explored, including a change in virulence of myxomatosis virus, infection with Rabbit Viral Haemorrhagic Disease 2 (RVHD2), or European Brown Hare Syndrome, individually or as co-infections, and it’s possible that other factors are involved.

What would really help the ongoing study into the large scale deaths of this iconic species would be for any members of the public finding a dead or ill hare to contact Dr Bell on: d.bell@uea.ac.uk. Please try and keep the body refrigerated whilst contacting Diana to arrange for a full post-mortem analysis.

Richard filming for our You Tube channel

Filming for RWAF YouTube channel

 

We are aware that our YouTube channel is in need of more content so we have recently spent the day with the lovely people at Vets4Pets Emmerson Green, Bristol, to film standard procedures and best practice. We hope that this will be accessible and useful to a wide range of people. Huge thanks to Sylvie Bolioli for giving up her time to do this

Watch this space for more videos in 2019

for us. We hope to have a lot of content for vets and owners in 2019. Again, watch this space for an update.

New RWAF Team member!

 

Baby Eden looks set to follow in her mums footsteps-(E Boyd)

We are excited to announce the newest member of the RWAF Team – please welcome baby Eden. Emma (Boyd) gave birth to gorgeous little Eden on 15th September. It will be no surprise to read that Eden is already a rabbit fan and has a good collection of rabbit themed clothes and toys. Emma is on maternity leave until the New Year but she will soon be back in the swing of things and working alongside Rae to organise the CPD for 2019. If Eden is anything like her amazing mum then animal welfare is going to have a fantastic new advocate.

RWAF Conference 2018

 

Langford Vet School, Bristol

23rd and 24th June

We are now taking bookings for our 2018 Conferences

 

RWAF Owner Conference 2018

2018 Owners pdf download details and booking form

The conference for owners and rescue workers can be booked here
https://shop.rabbitwelfare.co.uk/product/rwaf-owners-conference-2018/

RWAF Veterinary Conference 2018

The veterinary conference is open to vets, vet nurses and vet students and places can be booked here https://shop.rabbitwelfare.co.uk/product/rwaf-veterinary-conference-2018/

2018 Vets flyer download details and booking form

 

In each case scroll down the page to see everything that’s on offer.

Once again we are very happy to be able to offer two days to all delegates. On the first day the two conferences will be separate and then on the second day all delegates will be brought together for the behaviour and welfare day. You can book just the Saturday or just the Sunday or for a discount both days together.

If you prefer not to book online we can take your booking by phone at the usual number 0844 324 6090 between 11 and 3 on weekdays.

Don’t miss your chance, book early. The number of places will be limited.

 

RVHD2 Update November 2017

This supersedes earlier advice

Please also see ‘Further Reading’ page here https://rabbitwelfare.co.uk/rabbit-health/further-reading/rvhd-further-reading/  (Note added 25/06/18)

There are two strains of RVHD, known as RVHD1 and RVHD2.  Both strains are lethal and you must vaccinate to protect your rabbits.  RVHD2 has been in the UK since 2013 and over time appears to be overtaking classic RVHD as the primary strain.

RVHD vaccines are very effective. Your rabbits can currently be protected against RHD1 using the Nobivac Myxomatosis-RHD vaccine with a booster every 12 months. Both strains of RVHD are covered by vaccination any time from ten weeks (Filavac) or RHD2 can be prevented from 30 days (Eravac) of age. The separate RVHD2 vaccine is given every 6-12 months. You should consult your vet for the best combination currently available for your rabbit(s).

It’s very important to clean and disinfect anything that may be carrying the viruses, including water bottles, bowls, bedding and housing.  This means that boarding and rescue rabbits, even with up to date vaccinations, may potentially be a risk, and establishments should take suitable precautions, as should vets who may have infected rabbits brought to them for treatment. Anything that has been touched by an unknown rabbit should be thoroughly cleaned and treated with virucidal agents. In high risk situations foot covers or foot dips may be wise measures.

If you are about to obtain a young rabbit that hasn’t yet been vaccinated:

  • Follow the advice given in the vaccinations section
  • Don’t use second-hand hutches or equipment without finding out what happened to the previous occupant.

FAQ on Rabbit Viral Haemorrhagic Disease 1 and 2 Background:

For background, whilst the “classic” RVHD has been present in the UK for decades, variant RHVD (also known as RHVD2 or RHDV variant) was first noted in 2010 in France, and has subsequently been identified in the UK (OIE Technical Disease Cards, updated July 2015; Abrantes et al, 2012; Dalton et al, 2012; Westcott and Choudry, 2014). This virus has some differences from the classic RVHD. In particularly it may affect rabbits of any age, as opposed to RVHD1, which is rarely if ever seen in rabbits under 8-10 weeks of age.

It has also been reported that the variant gives rise to lower mortalities than classical RVHD, this is not necessarily borne out by reports (Abrantes et al, 2013), and this may be thought to be due to be the case due to its phylogenetic placement alongside non-pathogenic strains.

Mortality may vary from collection to collection, and possibly from breed to breed. The only vaccine for rabbits initially available with a UK License was Nobivac Myxo-RHD (MSD Animal Health), which was made available in 2012. Not long after that, the other 3 vaccines against RHVD on the UK market ceased to be available.

This vaccine does not appear to offer protection against RVHD2, and neither do the previous vaccine brands available in the UK. However, RHD1 and Myxo remained the most significant health threats which could be vaccinated against, and so coverage with this product remains a priority. Work from Italy and France, however, suggests that, with our reservoir of wild rabbits, we can expect to see RHD2 starting to predominate over RHD1 in the next 5 years or so.

Previously there were three vaccines (Filavac VHD K C+V, Cunivak RHD and Cunipravac RHD-2 Variant) that initially had a Special Import or Special Treatment Certificate from the Veterinary Medicines Directorate, on the basis of a clear need to do so given the current disease status. That need now having been firmly established, Filavac VHD K C+V, and Eravac VHD are now licensed. Practices should check availability of these products directly with their wholesalers. Owners should check with their practices which vaccines they hold, and what the protocol for vaccinating rabbits is at the practice in question.

Stocks are sometimes very variable, and practices are advised to contact wholesalers directly for information on stock availability. I would still be interested in any other practitioners findings regarding reports of suspected or confirmed RHD1 and 2.

 FAQs

Is vaccination necessary?

This will obviously involve a risk assessment of the individual rabbit(s), but the wide geographical range of the disease, and the reported losses of several hundred rabbits throughout the UK, as well as molecular testing confirmation of cause of death in many sampled, suggests that vaccination is strongly advisable.

Moredun Institute has advised RWAF that cases have been confirmed throughout the UK, so you cannot assume you are in a ‘safe’ area. Additionally we believe that RVHD2 will be significantly under reported. Because RHD2 doesn’t always look like classic RHD1, a rabbit could be taken into hospital looking ill, but nobody would necessarily think to treat that potentially infectious case for RHD2

Do existing RHD1 vaccines work? Because the mortality rate is lower with RHD2, any test using a small number of rabbits could easily show protection just because none of them were going to die anyway. There is some anecdotal evidence that RHD1 vaccines have some short term effect, but nothing peer reviewed. Le Gall-Recule (2013) showed that cross immunity between RHD1 and 2 was, at best, partial.

Do RHD2 vaccines work? Le Minor et al (2013) showed that Filavac produced good immunity (full protection) against RHD2 in challenge studies. (15èmes Journées de la Recherche Cunicole, 19-20 novembre 2013, Le Mans, France)

How will you get it from your vet?

Please only go through your vets, rather than contacting wholesalers directly.

What dose regime is suggested?

Standard advice with immunological products not licensed for simultaneous administration is to space them out by at least 2 weeks, and this is the regime we advise with both vaccines.

The duration of immunity for Filavac has been established at at least 12 months, in laboratory conditions in healthy rabbits. The manufacturer’s advice is to administer a single dose of the vaccine, followed by annual boosters in low risk situations, and 6 monthly in the case of breeding does at high risk. In the UK, I would suggest that high risk situations include rescue centres and breeders, unless they have a strict quarantine policy, and those rabbits which have greater contact with wild rabbits, as well as any geographical location where cases have been reported recently. All other rabbits are likely to fall into the lower risk category, requiring annual re-vaccination. The duration of immunity has not been confirmed in the case of Eravac, but this is expected to be updated shortly.

What does the vaccine cost?

Here at the RWAF we are not able to monitor or affect the prices charged by veterinary practices. It’s worth pointing out that the price of the vaccine may vary widely between practices due to pricing structures, and due to the caseload of rabbits that they see. If they are able to make use of larger vaccine vials, the cost may be shared across more rabbits and reduced, but this is not often possible, as it requires enough rabbits to be seen in a 2 hour window during which the vial may be used.

What if I buried my pet rabbit and now wonder if it was RVHD 2, will the ground be infected and a risk to my other rabbits? (How should bodies be disposed of?)

There is not enough information out there to know the correct answer to this. We know it can live for 200 days in ideal conditions, so there is in theory a potential risk but we are speculating here. The best way to dispose of the body of any rabbit that died a sudden or unexplained death is to ask your vet to get it cremated for you. Double wrap them in plastic, and disinfect the outside, before taking to your vet, to reduce the risk of disease spread.

Once rabbits have recovered from RVHD2 do they still carry it? Do they still shed? Can I bond to another rabbit safely without risking them?

There is not enough information known about RVHD2 to know the correct answer to this with any certainty. In theory they should be safe to bond after 200 days, in practice it may be safe sooner than this, but we really don’t know.

Can you recommend a cleaning protocol?

90% of any disinfection is cleaning, that is the most important aspect. After thorough cleaning of the area to remove any scale or residue, use Ark-Klens , which is a benzalkonium chloride disinfectant and as such it should be effective against EC and myxi, to routinely disinfect the housing. Periodically use Virkon (as an inorganic peroxygen compound) to kill any other viruses.   Anigene HLD4V has been confirmed as effective against RVHD2 at a dilution of 1:50. It is important that the correct dilution is used”.  Note: Other benzalkonium chloride disinfectants and inorganic peroxygen compounds may be available, in addition to those named above.

Other than vaccination can I prevent my rabbit getting RHD? Will they get it from hay?

They are very unlikely to get RHD (1 or 2) or Myxomatosis from hay or barn dried grass. Risk / benefit analysis would be in the favour of feeding these foods. Foraged foods may potentially carry RVHD. Try to obtain plants from areas out of the reach of wild rabbits, and do not collect forage from areas of known wild rabbit RVHD infection. Biosecurity advice was given in the webinar (link above) but summarised here: Use foot dips or change footwear between going outside, especially into areas frequented by wild rabbits Quarantine new animals, feed them last, use new equipment such as bottles / bowls for them. Barrier nurse any suspicious cases Try to exclude wild rabbits and unless they can be excluded from the garden consider stopping the practice of moving pens around the garden and even consider a double fence round rabbit runs.

What are the risks of “over-vaccination” and vaccine ingredients?

Vaccinating with an RHD 1 and 2 vaccine (Filavac), 1-2 times per year, on top of an existing RVHD1 and Myxomatosis vaccine (Nobivac), obviously increases the vaccine frequency and amount given to each rabbit. This is not perfect, but the alternative is missing out one of these vaccines, and the risk of “over-vaccination” is considered lower than the risk of insufficient protection. Filavac is an inactivated, adjuvanted vaccine, and so cannot lead to clinical RVHD in the animal. Eravac covers only RHD2, and so there is less concern about “over-vaccination” with this product. Concerns are often raised about vaccine ingredients (adjuvants and excipients) such as aluminium hydroxide and sodium metabisulphite. This is too large a topic to discuss here, but, without dismissing these concerns out of hand, and after weighing the risks against the benefits, vaccination has a strongly net positive benefit against the diseases discussed here. There are known vaccine side effects discussed in the data sheets for these vaccines. They are usually limited to small local transient skin reactions, and transient mild lethargy. Oil based vaccines such as Eravac carry a known risk of skin and subcutaneous tissue damage, and great care must be taken to ensure no vaccine enters the intradermal route, to minimise this risk, as well as taking care not to accidentally self-inject. The frequency of vaccination, and a risk:benefit analysis for each individual, should be discussed between client and veterinary surgeon before deciding on an appropriate regime and vaccination plan. There is a risk to any animal (or person) to having any vaccination, which is why animals (or people) should only be vaccinated if they are healthy. For further general details on companion animals, the BSAVA and WSAVA vaccine guidelines should be consulted. Note that under their definitions, in the UK and mainland Europe, RHD2 would be considered a “core” vaccination.

https://www.bsava.com/Resources/Positionstatements/Vaccination.aspx http://www.wsava.org/guidelines/vaccination-guidelines Titre testing against this strain is not commercially available, at least at present in the UK. It’s also worth being aware that other countries are slightly ahead of us in arranging vaccine importation and use for domestic rabbits. In Holland, vaccination has been underway with Filavac for several months before its use in the UK, and they also use the Nobivac Myxo-RHD vaccine.

Where can I send samples to get RVHD2 confirmed?

The following labs currently offer testing for RHD1 and 2. The OIE (World Organisation for Animal Health) guidelines suggest that liver is the best sample by far, but that spleen and blood are also good tissues to sample, as the virus becomes widespread throughout the body via the blood. The use of swabs to obtain samples from the tissues is possible, but to avoid false results, plain swabs (ie NOT in bacteriology medium such as agar gel or charcoal based material), with metal or plastic handles, not wood, should be used.

The use of faeces or urine samples or conjunctival swabs is less well evaluated, and we would not currently recommend testing via these methods when a test that is validated in the live animal (blood) is available

http://www.battlab.com/PCRTests.pdf

http://www.palsvetlab.co.uk

http://laboklin.co.uk/laboklin/showGeneralTest.jsp?testID=8518

 

Refs: Joana Abrantes, Wessel van der Loo, Jacques Le Pendu and Pedro J Esteves (2012) Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review Veterinary Research 2012, 43:12 doi:10.1186/1297-9716-43-12 Kevin P. Dalton, Inés Nicieza, Ana Balseiro, María A. Muguerza, Joan M. Rosell, Rosa Casais, Ángel L. Álvarez, and Francisco Parra(2012) Variant Rabbit Hemorrhagic Disease Virus in Young Rabbits, Spain Emerg Infect Dis. 2012 Dec; 18(12): 2009–2012. doi: 10.3201/eid1812.120341 D. G. Westcott and B. Choudhury Rabbit haemorrhagic disease virus 2-like variant in Great Britain Veterinary Record doi:10.1136/vr.102830 Joana Abrantes, Ana M. Lopes, Kevin P. Dalton, Pedro Melo, Jorge J. Correia, Margarida Ramada, Paulo C. Alves,Francisco Parra, and Pedro J. Esteves New Variant of Rabbit Hemorrhagic Disease Virus, Portugal, 2012–2013 Emerg Infect Dis. 2013 Nov; 19(11): 1900–1902. doi: 10.3201/eid1911.130908 Detection of a new variant of rabbit haemorrhagic disease virus in France G. Le Gall-Reculé et al February 5, 2011 | Veterinary Record | 137-138 doi: 10.1136/vr.d697 Emergence of a new lagovirus related to Rabbit Haemorrhagic Disease Virus Ghislaine Le Gall-Reculé et al (2013) Veterinary Research 2013 44:81 DOI: 10.1186/1297-9716-44-81

Other useful sources of information: http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1005087 http://www.iucn-whsg.org/RabbitHemorrhagicDiseaseInEurope https://www.harcourt-brown.co.uk/articles/infectious-disease/rabbit-haemorrhagic-disease Webinar: http://therabbitvet.com/webinar/vhd-rhd-2-update-rabbit-haemorrhagic-disease

RVHD2 update, August 2017

 For the latest advice please see this post https://rabbitwelfare.co.uk/rvhd2-update-november-2017/

Cases of new variant rabbit haemorrhagic disease (RHD2) continue to be reported on a regular basis. Several laboratories will undertake PCR testing for RHD2 and it is now possible to test samples from live animals, making it quicker and easier to diagnose and more likely to get owner agreement for testing. However, these are PCR tests which depend on the virus being present in the blood or shed into urine and faeces, and so a negative is not conclusive, although a positive is. Liver remains the best sample for testing in the post mortem rabbit.

During 2016, in the absence of an effective UK-licenced vaccine to protect against RHD2, we arranged for the import of a number of RHD2 vaccines from around Europe. Filavac VHD K C+V, manufactured by Filavie, has been the main vaccine imported, primarily due to its proven efficacy, safety and availability in single dose vials. In the ten months Filavac was being imported through the Special Import scheme, 3420 special import certificates (SIC) were granted, making it the second most granted SIC over that period. Based on an earlier Freedom of Information Act request, we estimate that this accounts for nearly 100,000 doses of the vaccine, almost certainly making it the most imported medicine of 2016 by some significant margin.

In October 2016, Eravac RHD2 became the first RHD2 vaccine to receive an EU-wide licence, making it available in the UK without the need for an import licence. This has now been followed by Filavac, which received its UK licence in April 2017. The fact that two separate companies have gone to the trouble and expense of getting their products licenced indicates that they believe there to be sufficient clinical need and ongoing product demand to make this investment worthwhile.

We anticipate that Filavac will become the main product used in UK veterinary practices due to its availability in single dose vials and information on efficacy and vaccination protocol for pet rabbits, requiring only a single booster every 12m in normal circumstances. Eravac is only available in multi-dose vials, and the vaccine is oil adjuvanted, presenting a risk to the user in the event of accidental self-injection. Its vaccination protocol and efficacy data is also less clear from its EU authorisation, with a booster currently recommended by the manufacturer every 6 months.

While in most circumstances we believe that Filavac is the more appropriate choice, it is still not perfect for use in the UK alongside Nobivac myxo-rhd, the only licenced recombinant vaccine against myxomatosis and classic RHD. The Filavac is not only effective against RHD2; it is a dual vaccine which also includes protection against classic RHD. This means that rabbits will receive duplicate vaccines against classic RHD when they are vaccinated according to the protocols for each product. While the benefits are thought to outweigh the risks, it is nevertheless not ideal. Multiple vet visits is also more likely to reduce owner compliance. The ideal situation would be the development of a single vaccine incorporating protection against myxomatosis, classic RHD and RHD2, allowing full protection without multiple visits or duplication of agents. Alternatively, a vaccine combining myxomatosis with RHD2 rather than classic RHD would provide cover for the increasingly prevalent and dominant strain reported in the UK.All recent reports of strain specific testing for RHD received by the RWAF have been for type 2, and so it is concluded that this has become the most prevalent virus strain now.

Now there are two RHD2 vaccines authorised for use in the UK, our priority is to continue raising awareness of RHD2 and the availability/selection of vaccines among veterinary professionals and pharmaceutical companies. We believe that RHD2 is a real and continuing threat to pet rabbits and this view is validated by two companies now producing an RHD2 vaccine for the UK market. We therefore aim to ensure that the UK’s pet rabbits are protected against RHD2 as well as myxomatosis and classic RHD in the most effective and straightforward manner, ideally in a single vaccine to maximise owner compliance and minimise rabbit stress.

In the past few years, anecdotally we have also observed an increasing number of reports of rabbits vaccinated with Nobivac myxo-rhd contracting serious cases of myxomatosis, with more than may be expected dying as a result. Whether this is a result of mutating strains which are not covered as effectively by the existing vaccine, or whether there are other reasons (such as latent RHD2 infection) for insufficient immune response is not yet understood. If further work is to be undertaken to create a new recombinant vaccine incorporating RHD2, would urge that the efficacy of the myxomatosis element of the vaccine be considered as part of this review.

 

Please also read our post from January on RVHD2 & Filavac with FAQs

RVHD2 & Filavac with FAQs

RVHD 2 – FAQ – updated January 2017

Please also see this update (August 2017)

We are pleased to note that it is now possible to order Filavac VHD K C + V from 3 UK wholesalers, NVS, Centaur and Henry Schein Animal Health Although there have been some teething problems with establishing the supply chain and there are currently some shortages, everything is now in place and the high demand from rabbit owners ought to be able to be met in the next few weeks. There was a lot of media activity following an interview that I did with The Telegraph in July 2016, and lots of vets are reporting an increase in demand for this new vaccine.

We are being asked by rabbit owners many times every day which vets stock the vaccine as their own doesn’t. Obviously we don’t know the answer to this, but it indicates that some practices may be losing rabbit owning clients if they don’t, so if you are one of those practices we urge you to consider stocking it. If you have confirmed RVHD2 cases, or cases that are suspected of being RVHD in rabbits up to date with their Nobivac vaccination, as well as informing MSD, we’d be grateful if you would let us know please, and ask the owner to contact one or both of the two Facebook groups tracking the spread of reported cases of RVHD. These are https://www.facebook.com/groups/MyxomatosismapfortheUK/ which is using a map to show where reported cases have occurred and a newer group https://www.facebook.com/groups/1744958082388756/ – you can also post there if you have the vaccine in stock to let owners know.

Because we have received a large volume of questions from vets about both the vaccine and the illness below is information from a document compiled by our Specialist Veterinary Adviser Mr Richard Saunders BSc (Hons), BVSc, MSB, CBiol, DZooMed (Mammalian), MRCVS, RCVS Specialist in Zoo and Wildlife Medicine . Questions from clients may be covered in this webinar given by Mr Saunders http://therabbitvet.com/webinar/vhdrhd-2-update-rabbit-haemorrhagic-disease

You are invited also to join our Facebook group for vets and VNs This is a closed group and you will be asked for your registration number with RCVS in order to gain membership.
https://www.facebook.com/groups/115492551808078/

Our next RWAF Veterinary Conference will be at the Royal (Dick) School of Veterinary Studies in Edinburgh. We will send further details in future emails. We also intend to begin a programme of roadshows where we will send experts to various parts of the country to work hands-on with vets and VNs on identified topics. We have been asked already about airways management and dental treatment of rabbits and also about CT scanning. These sessions will be with small groups of approximately10 professionals so that they can be given proper attention and will they will receive CPD certification. We may invite a group of practices to send vets or VNs, or we may work with a single practice, depending on demand. We will be able to give fuller details soon.

FAQ on Rabbit Viral Haemorrhagic Disease 1 and 2

Background:

For background, whilst the “classic” RVHD has been present in the UK for decades, variant RHVD (also known as RHVD2 or RHDV variant) was first noted in 2010 in France, and has subsequently been identified in the UK (OIE Technical Disease Cards, updated July 2015; Abrantes et al, 2012; Dalton et al, 2012; Westcott and Choudry, 2014).

This virus has some differences from the classic RVHD. In particularly it may affect rabbits of any age, as
opposed to RVHD1, which is rarely if ever seen in rabbits under 8-10 weeks of age. It has also been reported that the variant gives rise to lower mortalities than classical RVHD, this is not necessarily borne out by reports (Abrantes et al, 2013), and this may be thought to be due to be the case due to its phylogenetic placement alongside non-pathogenic strains. Mortality may vary from collection to collection, and possibly from breed to
breed.

The only vaccine for rabbits currently available with a UK License is Nobivac Myxo-RHD (MSD Animal Health), which was made available in 2012. Not long after that, the other 3 vaccines against RHVD on the UK market ceased to be available.

This vaccine does not appear to offer protection against RVHD2, and neither do the previous vaccine brands available in the UK. However, RHD1 and Myxo remain the most significant health threats which can be vaccinated against, and so coverage with this product remains a priority.

Work from Italy and France, however, suggests that, with our reservoir of wild rabbits, we can expect to see RHD2 starting to predominate over RHD1 in the next 5 years or so.

However, there are now 4 vaccines available in the EU which have been licensed or are undergoing licensing for efficacy against RVHD2.

Three of these vaccines (Filavac VHD K C+V, Cunivak RHD and Cunipravac RHD-2 Variant) now have a Special Import or Special Treatment Certificate from the Veterinary Medicines Directorate, on the basis of a clear need to do so given the current disease status.

In particular, Filavac VHD K C+V is available through a UK wholesaler, precluding the need to order it directly from France, but note that the veterinary practice ordering it still needs to obtain an SIC from the VMD. At present, stocks are available through three wholesalers, NVS, Henry Schein, and Centaur, but availability is very variable, and practices are advised to contact wholesalers directly for information on stock availability. There is no reason why other wholesalers cannot stock this product, and practices tied to a specific wholesaler may want to consider encouraging them to stock it.

The Cunivak RHD is no longer available, and we do not anticipate re-ordering this product.

The Cunipravac may be obtained by ordering directly from the manufacturers. However, it is only available in relatively large vial sizes, making it impractical for practice use.

I would still be interested in any other practitioners findings regarding this situation, in particular whether they have seen dead or dying rabbits with suspected RVHD1 and/or 2, especially if they have gross PM, histopathology and, especially differential testing as performed by the Moredun Institute, Edinburgh.

This webinar may be of interest:
http://therabbitvet.com/webinar/vhd-rhd-2-update-rabbit-haemorrhagic-disease

Richard Saunders
Veterinary Adviser, Rabbit Welfare Association and Fund, Enigma House, Culmhead Business Centre, Taunton, Somerset TA3 7DY

Refs:

Joana Abrantes, Wessel van der Loo, Jacques Le Pendu and Pedro J Esteves (2012)
Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review
Veterinary Research 2012, 43:12 doi:10.1186/1297-9716-43-12

Kevin P. Dalton, Inés Nicieza, Ana Balseiro, María A. Muguerza, Joan M. Rosell, Rosa Casais, Ángel L. Álvarez, and Francisco Parra(2012)
Variant Rabbit Hemorrhagic Disease Virus in Young Rabbits, Spain
Emerg Infect Dis. 2012 Dec; 18(12): 2009–2012.
doi: 10.3201/eid1812.120341

D. G. Westcott and B. Choudhury
Rabbit haemorrhagic disease virus 2-like variant in Great Britain

Veterinary Record doi:10.1136/vr.102830

Joana Abrantes, Ana M. Lopes, Kevin P. Dalton, Pedro Melo, Jorge J. Correia, Margarida Ramada, Paulo C. Alves,Francisco Parra, and Pedro J. Esteves
New Variant of Rabbit Hemorrhagic Disease Virus, Portugal, 2012–2013
Emerg Infect Dis. 2013 Nov; 19(11): 1900–1902.
doi: 10.3201/eid1911.130908

Detection of a new variant of rabbit haemorrhagic disease virus in France
G. Le Gall-Reculé et al
February 5, 2011 | Veterinary Record | 137-138
doi: 10.1136/vr.d697

Emergence of a new lagovirus related to Rabbit Haemorrhagic Disease Virus
Ghislaine Le Gall-Reculé et al (2013)
Veterinary Research 2013 44:81
DOI: 10.1186/1297-9716-44-81

Other useful sources of information:

http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1005087

http://www.iucn-whsg.org/RabbitHemorrhagicDiseaseInEurope

https://www.harcourt-brown.co.uk/articles/infectious-disease/rabbit-haemorrhagic-disease

Webinar: http://therabbitvet.com/webinar/vhd-rhd-2-update-rabbit-haemorrhagic-disease

FAQs

Is vaccination necessary?

This will obviously involve a risk assessment of the individual rabbit(s), but the wide geographical range of the disease, and the reported losses of several hundred rabbits throughout the UK, as well as molecular testing confirmation of cause of death in many sampled, suggests that vaccination is strongly advisable. Moredun Institute has advised RWAF that cases have been confirmed throughout the UK, so you cannot assume you are in a ‘safe’ area. Additionally we believe that RVHD2 will be significantly under reported. Because RHD2 doesn’t always look like classic RHD1, a rabbit could be taken into hospital looking ill, but nobody would necessarily think to treat that potentially infectious case for RHD2

Do existing RHD1 vaccines work?

Because the mortality rate is lower with RHD2, any test using a small number of rabbits could easily show
protection just because none of them were going to die anyway. There is some anecdotal evidence that RHD1 vaccines have some short term effect, but nothing peer reviewed. Le Gall-Recule (2013) showed that cross immunity between RHD1 and 2 was, at best, partial.

Do RHD2 vaccines work?

Le Minor et al (2013) showed that Filavac produced good immunity (full protection) against RHD2 in challenge studies. (15èmes Journées de la Recherche Cunicole, 19-20 novembre 2013, Le Mans, France)

How will you get it from your vet?

Please only go through your vets, rather than contacting wholesalers directly. The wholesalers will be
overwhelmed with requests for information otherwise, and it cannot be obtained directly from them in any case. Your vets will need their own licence, which, now all the info is on the VMD site and is approved, should be straightforward to do. However, this is not as simple as writing a prescription, and your vet may not see enough rabbits for this to be a practical option for them.

What dose regime is suggested?

Please remember that the use of these products is both off licence (although under the Cascade), and subject to the VMD’s directions on importation of immunological products. As a result, although the manufacturers of the Filavac product suggest that vaccination can be at the same time as the Nobivac RHD-Myxo, as long as it is not in the same site or the same syringe, standard advice with immunological products not licensed for simultaneous administration is to space them out by at least 2 weeks.

The duration of immunity has been established at at least 12 months, in laboratory conditions in healthy rabbits.

The manufacturer’s advice is to administer a single dose of the vaccine, followed by annual boosters in low risk situations, and 6 monthly in the case of breeding does at high risk. In the UK, I would suggest that high risk situations include rescue centres and breeders, unless they have a strict quarantine policy, and those rabbits which have greater contact with wild rabbits, as well as any geographical location where cases have been reported recently. All other rabbits are likely to fall into the lower risk category, requiring annual re-vaccination.

What does the vaccine cost?

Here at the RWAF we are not able to monitor or affect the prices charged by veterinary practices. It’s worth pointing out that the price of the vaccine may vary widely between practices due to pricing structures, and due to the caseload of rabbits that they see. If they are able to make use of larger vaccine vials, the cost may be shared across more rabbits and reduced, but this is not often possible, as it requires enough rabbits to be seen in a 2 hour window during which the vial may be used.

What if I buried my pet rabbit and now wonder if it was RVHD 2, will the ground be infected and a risk to
my other rabbits? (How should bodies be disposed of?)

There is not enough information out there to know the correct answer to this. We know it can live for 200 days in ideal conditions, so there is in theory a potential risk but we are speculating here. The best way to dispose of the body of any rabbit that died a sudden or unexplained death is to ask your vet to get it cremated for you. Double wrap them in plastic, and disinfect the outside, before taking to your vet, to reduce the risk of disease spread.

Once rabbits have recovered from RVHD2 do they still carry it? Do they still shed? Can I bond to another rabbit safely without risking them?

There is not enough information known about RVHD2 to know the correct answer to this with any certainty. In theory they should be safe to bond after 200 days, in practice it may be safe sooner than this, but we really don’t know.

Can you recommend a cleaning protocol?

90% of any disinfection is cleaning, that is the most important aspect. After thorough cleaning of the area to remove any scale or residue, use Ark-Klens , which is a benzalkonium chloride disinfectant and as such it should be effective against EC and myxi, to routinely disinfect the housing. Periodically use Virkon (as an inorganic peroxygen compound) to kill any other viruses.

Anigene HLD4V has been confirmed as effective against RVHD2 at a dilution of 1:50. It is important that the correct dilution is used.

Note: Other benzalkonium chloride disinfectants and inorganic peroxygen compounds may be available, in addition to those named above.

Other than vaccination can I prevent my rabbit getting RHD? Will they get it from hay?

They are very unlikely to get RHD (1 or 2) or Myxomatosis from hay or barn dried grass. Risk / benefit analysis would be in the favour of feeding these foods. Foraged foods may potentially carry RVHD. Try to obtain plants from areas out of the reach of wild rabbits, and do not collect forage from areas of known wild rabbit RVHD infection.

Biosecurity advice was given in the webinar (link above) but summarised here:

Use foot dips or change footwear between going outside, especially into areas frequented by wild rabbits
Quarantine new animals, feed them last, use new equipment such as bottles / bowls for them.
Barrier nurse any suspicious cases

Try to exclude wild rabbits and unless they can be excluded from the garden consider stopping the practice of moving pens around the garden and even consider a double fence round rabbit runs.

What are the risks of “over-vaccination” and vaccine ingredients?

Vaccinating with an RHD 1 and 2 vaccine (Filavac), 1-2 times per year, on top of an existing RVHD1 and Myxomatosis vaccine (Nobivac), obviously increases the vaccine frequency and amount given to each rabbit.
This is not perfect, but the alternative is missing out one of these vaccines, and the risk of “over-vaccination” is considered lower than the risk of insufficient protection.

Filavac is an inactivated, adjuvanted vaccine, and so cannot lead to clinical RVHD in the animal.

Concerns are often raised about vaccine ingredients (adjuvants and excipients) such as aluminium hydroxide and sodium metabisulphite. This is too large a topic to discuss here, but, without dismissing these concerns out of hand, and after weighing the risks against the benefits, vaccination has a strongly net positive benefit against the diseases discussed here.

There are known vaccine side effects discussed in the data sheets for these vaccines. They are usually limited to small local transient skin reactions, and transient mild lethargy. Oil based vaccines such as Cunipravac RHD2 Variant carry a known risk of significant skin and subcutaneous tissue damage, and great care must be taken to ensure no vaccine enters the intradermal route, to minimise this risk.

The frequency of vaccination, and a risk:benefit analysis for each individual, should be discussed between client and veterinary surgeon before deciding on an appropriate regime and vaccination plan. There is a risk to any animal (or person) to having any vaccination, which is why animals (or people) should only be vaccinated if they are healthy.

For further general details on companion animals, the BSAVA and WSAVA vaccine guidelines should be consulted. Note that under their definitions, in the UK and mainland Europe, RHD2 would be considered a “core” vaccination.

https://www.bsava.com/Resources/Positionstatements/Vaccination.aspx

http://www.wsava.org/guidelines/vaccination-guidelines

Titre testing against this strain is not commercially available, at least at present in the UK.

It’s also worth being aware that other countries are slightly ahead of us in arranging vaccine importation and use for domestic rabbits. In Holland, vaccination has been underway with Filavac for several months before its use in the UK, and they also use the Nobivac Myxo-RHD vaccine.

Where can I send samples to get RVHD2 confirmed?

The following labs currently offer testing for RHD1 and 2. The OIE (World Organisation for Animal Health) guidelines suggest that liver is the best sample by far, but that spleen and blood are also good tissues to sample, as the virus becomes widespread throughout the body via the blood. The use of swabs to obtain samples from the tissues is possible, but to avoid false results, plain swabs (ie NOT in bacteriology medium such as agar gel or charcoal based material), with metal or plastic handles, not wood, should be used.

The use of faeces or urine samples or conjunctival swabs is less well evaluated, and we would not currently recommend testing via these methods when a test that is validated in the live animal (blood) is available

http://www.battlab.com/PCRTests.pdf

http://www.palsvetlab.co.uk

http://laboklin.co.uk/laboklin/showGeneralTest.jsp?testID=8518

Batt Laboratories, University of Warwick Science Park, The Venture Centre
Sir William Lyons Road, Coventry, CV4 7EZ
Tel: 0247 6323275 Fax: 0871 7505323

Testing on tissue samples for Rabbit Haemorrhagic Disease. The PCR has a price of £45 and includes differentiation between RHDV1 and RHDV2. For live animals we recommend conjunctival swabs, urine and/or faeces. Blood is also possible, but mainly in case of suspect viraemia. For dead animals liver sample can also be submitted (without formalin). Turnaround time is 2-4 working days.

We offer RHDV test (conventional PCR) followed by sequencing for RHDV1/RHDV2 discrimination.

We run the test whenever is required, we don’t have specified turnaround time, however there is the option to run it with urgency if you (or another clinician) suspects an outbreak. In this case the best option is to e-mail or phone me directly, so I can confirm the likely test date.

 

What about Epravac?

“The recent licensing of EPRAVAC RHD2 vaccine for use in the UK is important in that it further recognises the concerns of the regulatory authorities and drug companies that RHD2 is a serious health and welfare concern to UK rabbits. However, there are a few caveats here, related to its origin as a vaccine for meat rabbits. The vaccine duration of action has not been determined, as meat rabbits are typically slaughtered very early in life. In fact, the product characteristics state: “Vaccinate only fattening rabbits. No information is available on the safety and efficacy in other categories such as breeding or pet rabbits.”

In addition, the vaccine is oil adjuvanted, necessitating the following user warning:

*Eravac is an emulsion containing mineral oil. Accidental injection may cause severe pain and selling, particularly if injected into a joint or finger – this could result in the loss of the finger if prompt medical attention is not given. If someone is accidentally injected with this product, they must seek medical attention immediately even if only a very small amount is injected. The package leaflet should be shown to the doctor. If pain persists for more than 12 hours after medical examination, the doctor should be contacted again.*

For these reasons, we feel that other vaccines, such as Filavac, covering RHD2 are preferable for the pet and rabbit population in the UK. As ever, rabbit owners are urged to discuss the specifics of their rabbit’s care with their own vets, and those vets are welcome to contact RWAF for further discussion should they wish to.”

RVHD2 – FAQ updated

 

Please also see this update (August 2017)

FAQ on Rabbit Viral Haemorrhagic Disease 1 and 2 Background:

For background, whilst the “classic” RVHD has been present in the UK for decades, variant RHVD (also known as RHVD2 or RHDV variant) was first noted in 2010 in France, and has subsequently been identified in the UK (OIE Technical Disease Cards, updated July 2015; Abrantes et al, 2012; Dalton et al, 2012; Westcott and Choudry, 2014). This virus has some differences from the classic RVHD. In particularly it may affect rabbits of any age, as opposed to RVHD1, which is rarely if ever seen in rabbits under 8-10 weeks of age.

It has also been reported that the variant gives rise to lower mortalities than classical RVHD, this is not necessarily borne out by reports (Abrantes et al, 2013), and this may be thought to be due to be the case due to its phylogenetic placement alongside non-pathogenic strains.

Mortality may vary from collection to collection, and possibly from breed to breed. The only vaccine for rabbits currently available with a UK License is Nobivac Myxo-RHD (MSD Animal Health), which was made available in 2012. Not long after that, the other 3 vaccines against RHVD on the UK market ceased to be available.

This vaccine does not appear to offer protection against RVHD2, and neither do the previous vaccine brands available in the UK. However, RHD1 and Myxo remain the most significant health threats which can be vaccinated against, and so coverage with this product remains a priority. Work from Italy and France, however, suggests that, with our reservoir of wild rabbits, we can expect to see RHD2 starting to predominate over RHD1 in the next 5 years or so.

However, there are now 4 vaccines available in the EU which have been licensed or are undergoing licensing for efficacy against RVHD2. Three of these vaccines (Filavac VHD K C+V, Cunivak RHD and Cunipravac RHD-2 Variant) now have a Special Import or Special Treatment Certificate from the Veterinary Medicines Directorate, on the basis of a clear need to do so given the current disease status. In particular, Filavac VHD K C+V is available through a UK wholesaler, precluding the need to order it directly from France, but note that the veterinary practice ordering it still needs to obtain an SIC from the VMD.

At present, stocks are available through three wholesalers, NVS, Henry Schein, and Centaur, but availability is very variable, and practices are advised to contact wholesalers directly for information on stock availability. There is no reason why other wholesalers cannot stock this product, and practices tied to a specific wholesaler may want to consider encouraging them to stock it. The Cunivak RHD is no longer available, and we do not anticipate re-ordering this product. The Cunipravac may be obtained by ordering directly from the manufacturers. However, it is only available in relatively large vial sizes, making it impractical for practice use. I would still be interested in any other practitioners findings regarding this situation, in particular whether they have seen dead or dying rabbits with suspected RVHD1 and/or 2, especially if they have gross PM, histopathology and, especially differential testing as performed by the Moredun Institute, Edinburgh.

 FAQs

Is vaccination necessary?

This will obviously involve a risk assessment of the individual rabbit(s), but the wide geographical range of the disease, and the reported losses of several hundred rabbits throughout the UK, as well as molecular testing confirmation of cause of death in many sampled, suggests that vaccination is strongly advisable. Moredun Institute has advised RWAF that cases have been confirmed throughout the UK, so you cannot assume you are in a ‘safe’ area. Additionally we believe that RVHD2 will be significantly under reported. Because RHD2 doesn’t always look like classic RHD1, a rabbit could be taken into hospital looking ill, but nobody would necessarily think to treat that potentially infectious case for RHD2 Do existing RHD1 vaccines work? Because the mortality rate is lower with RHD2, any test using a small number of rabbits could easily show protection just because none of them were going to die anyway. There is some anecdotal evidence that RHD1 vaccines have some short term effect, but nothing peer reviewed. Le Gall-Recule (2013) showed that cross immunity between RHD1 and 2 was, at best, partial. Do RHD2 vaccines work? Le Minor et al (2013) showed that Filavac produced good immunity (full protection) against RHD2 in challenge studies. (15èmes Journées de la Recherche Cunicole, 19-20 novembre 2013, Le Mans, France)

How will you get it from your vet?

Please only go through your vets, rather than contacting wholesalers directly. The wholesalers will be overwhelmed with requests for information otherwise, and it cannot be obtained directly from them in any case. Your vets will need their own licence, which, now all the info is on the VMD site and is approved, should be straightforward to do. However, this is not as simple as writing a prescription, and your vet may not see enough rabbits for this to be a practical option for them.

What dose regime is suggested?

Please remember that the use of these products is both off licence (although under the Cascade), and subject to the VMD’s directions on importation of immunological products. As a result, although the manufacturers of the Filavac product suggest that vaccination can be at the same time as the Nobivac RHD-Myxo, as long as it is not in the same site or the same syringe, standard advice with immunological products not licensed for simultaneous administration is to space them out by at least 2 weeks. The duration of immunity has been established at at least 12 months, in laboratory conditions in healthy rabbits. The manufacturer’s advice is to administer a single dose of the vaccine, followed by annual boosters in low risk situations, and 6 monthly in the case of breeding does at high risk. In the UK, I would suggest that high risk situations include rescue centres and breeders, unless they have a strict quarantine policy, and those rabbits which have greater contact with wild rabbits, as well as any geographical location where cases have been reported recently. All other rabbits are likely to fall into the lower risk category, requiring annual re-vaccination.

What does the vaccine cost?

Here at the RWAF we are not able to monitor or affect the prices charged by veterinary practices. It’s worth pointing out that the price of the vaccine may vary widely between practices due to pricing structures, and due to the caseload of rabbits that they see. If they are able to make use of larger vaccine vials, the cost may be shared across more rabbits and reduced, but this is not often possible, as it requires enough rabbits to be seen in a 2 hour window during which the vial may be used.

What if I buried my pet rabbit and now wonder if it was RVHD 2, will the ground be infected and a risk to my other rabbits? (How should bodies be disposed of?)

There is not enough information out there to know the correct answer to this. We know it can live for 200 days in ideal conditions, so there is in theory a potential risk but we are speculating here. The best way to dispose of the body of any rabbit that died a sudden or unexplained death is to ask your vet to get it cremated for you. Double wrap them in plastic, and disinfect the outside, before taking to your vet, to reduce the risk of disease spread.

Once rabbits have recovered from RVHD2 do they still carry it? Do they still shed? Can I bond to another rabbit safely without risking them?

There is not enough information known about RVHD2 to know the correct answer to this with any certainty. In theory they should be safe to bond after 200 days, in practice it may be safe sooner than this, but we really don’t know.

Can you recommend a cleaning protocol?

90% of any disinfection is cleaning, that is the most important aspect. After thorough cleaning of the area to remove any scale or residue, use Ark-Klens , which is a benzalkonium chloride disinfectant and as such it should be effective against EC and myxi, to routinely disinfect the housing. Periodically use Virkon (as an inorganic peroxygen compound) to kill any other viruses. Note: Other benzalkonium chloride disinfectants and inorganic peroxygen compounds may be available, in addition to those named above.

Other than vaccination can I prevent my rabbit getting RHD? Will they get it from hay?

They are very unlikely to get RHD (1 or 2) or Myxomatosis from hay or barn dried grass. Risk / benefit analysis would be in the favour of feeding these foods. Foraged foods may potentially carry RVHD. Try to obtain plants from areas out of the reach of wild rabbits, and do not collect forage from areas of known wild rabbit RVHD infection. Biosecurity advice was given in the webinar (link above) but summarised here: Use foot dips or change footwear between going outside, especially into areas frequented by wild rabbits Quarantine new animals, feed them last, use new equipment such as bottles / bowls for them. Barrier nurse any suspicious cases Try to exclude wild rabbits and unless they can be excluded from the garden consider stopping the practice of moving pens around the garden and even consider a double fence round rabbit runs.

What are the risks of “over-vaccination” and vaccine ingredients?

Vaccinating with an RHD 1 and 2 vaccine (Filavac), 1-2 times per year, on top of an existing RVHD1 and Myxomatosis vaccine (Nobivac), obviously increases the vaccine frequency and amount given to each rabbit. This is not perfect, but the alternative is missing out one of these vaccines, and the risk of “over-vaccination” is considered lower than the risk of insufficient protection. Filavac is an inactivated, adjuvanted vaccine, and so cannot lead to clinical RVHD in the animal. Concerns are often raised about vaccine ingredients (adjuvants and excipients) such as aluminium hydroxide and sodium metabisulphite. This is too large a topic to discuss here, but, without dismissing these concerns out of hand, and after weighing the risks against the benefits, vaccination has a strongly net positive benefit against the diseases discussed here. There are known vaccine side effects discussed in the data sheets for these vaccines. They are usually limited to small local transient skin reactions, and transient mild lethargy. Oil based vaccines such as Cunipravac RHD2 Variant carry a known risk of significant skin and subcutaneous tissue damage, and great care must be taken to ensure no vaccine enters the intradermal route, to minimise this risk. The frequency of vaccination, and a risk:benefit analysis for each individual, should be discussed between client and veterinary surgeon before deciding on an appropriate regime and vaccination plan. There is a risk to any animal (or person) to having any vaccination, which is why animals (or people) should only be vaccinated if they are healthy. For further general details on companion animals, the BSAVA and WSAVA vaccine guidelines should be consulted. Note that under their definitions, in the UK and mainland Europe, RHD2 would be considered a “core” vaccination. https://www.bsava.com/Resources/Positionstatements/Vaccination.aspx http://www.wsava.org/guidelines/vaccination-guidelines Titre testing against this strain is not commercially available, at least at present in the UK. It’s also worth being aware that other countries are slightly ahead of us in arranging vaccine importation and use for domestic rabbits. In Holland, vaccination has been underway with Filavac for several months before its use in the UK, and they also use the Nobivac Myxo-RHD vaccine.

Refs: Joana Abrantes, Wessel van der Loo, Jacques Le Pendu and Pedro J Esteves (2012) Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review Veterinary Research 2012, 43:12 doi:10.1186/1297-9716-43-12 Kevin P. Dalton, Inés Nicieza, Ana Balseiro, María A. Muguerza, Joan M. Rosell, Rosa Casais, Ángel L. Álvarez, and Francisco Parra(2012) Variant Rabbit Hemorrhagic Disease Virus in Young Rabbits, Spain Emerg Infect Dis. 2012 Dec; 18(12): 2009–2012. doi: 10.3201/eid1812.120341 D. G. Westcott and B. Choudhury Rabbit haemorrhagic disease virus 2-like variant in Great Britain Veterinary Record doi:10.1136/vr.102830 Joana Abrantes, Ana M. Lopes, Kevin P. Dalton, Pedro Melo, Jorge J. Correia, Margarida Ramada, Paulo C. Alves,Francisco Parra, and Pedro J. Esteves New Variant of Rabbit Hemorrhagic Disease Virus, Portugal, 2012–2013 Emerg Infect Dis. 2013 Nov; 19(11): 1900–1902. doi: 10.3201/eid1911.130908 Detection of a new variant of rabbit haemorrhagic disease virus in France G. Le Gall-Reculé et al February 5, 2011 | Veterinary Record | 137-138 doi: 10.1136/vr.d697 Emergence of a new lagovirus related to Rabbit Haemorrhagic Disease Virus Ghislaine Le Gall-Reculé et al (2013) Veterinary Research 2013 44:81 DOI: 10.1186/1297-9716-44-81

Other useful sources of information: http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1005087 http://www.iucn-whsg.org/RabbitHemorrhagicDiseaseInEurope https://www.harcourt-brown.co.uk/articles/infectious-disease/rabbit-haemorrhagic-disease Webinar: http://therabbitvet.com/webinar/vhd-rhd-2-update-rabbit-haemorrhagic-disease